Preparation of a plasma membrane-rich fraction from rat spermatozoa
نویسندگان
چکیده
منابع مشابه
Preparation and Characterization of a Plasma Membrane Fraction from Isolated Fat Cells
A rapid method of preparing plasma membranes from isolated fat cells is described. After homogenization of the cells, various fractions were isolated by differential centrifugation and linear gradients. Ficoll gradients were preferred because total preparation time was under 3 hr. The density of the plasma membranes was 1.14 in sucrose. The plasma membrane fraction was virtually uncontaminated ...
متن کاملIsolation of a Golgi Apparatus-rich Fraction from Rat Liver
Golgi apparatus were released without fixatives from rat hepatocytes by gentle homogenization, concentrated by differential centrifugation, and purified by sucrose gradient centrifugation. Examination of sections of purified fractions by electron microscopy showed fields of morphologically intact units of Golgi apparatus consisting of stacks of parallel flattened cisternae, secretory vesicles, ...
متن کاملPreparation of Plasma Membrane from Isolated Neurons
A bulk fraction enriched with respect to neuronal cell bodies was used as starting material for the isolation of neuronal plasma membrane The cells were gently homogenized in isotonic sucrose and a crude membrane containing fraction sedimented at 3000 g. Subsequently, the membrane fraction was purified on a discontinuous sucrose density gradient between 35% and 25 5% sucrose (w/w). Enzymatic an...
متن کاملPlasma membrane of the rat parotid gland: preparation and partial characterization of a fraction containing the secretory surface
A plasma membrane fraction from the rat parotid gland has been prepared by a procedure which selectively enriches for large membrane sheets. This fraction appears to have preserved several ultrastructural features of the acinar cell surface observed in situ. Regions of membrane resembling the acinar luminal border appear as compartments containing microvillar invaginations, bounded by elements ...
متن کاملProperdin: preparation from plasma fraction I of the Cohn method.
Pillemer et al. (I) have shown that normal human and other mammalian serums contain a trace protein which has been called properdin. Properdin, in conjunction with complement and YIg++, participates in the natural defense mechanism of blood (2). Pillemer et al. (1) have described a method for preparing properdin from serum. This method employs zymosan to adsorb the properdin, the trace protein ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Reproduction
سال: 1986
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0770435